iForest - Biogeosciences and Forestry


Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings

Aleksandar Vemić (1), Vladan Popović (2)   , Zoran Miletić (3), Zlatan Radulović (4), Ljubinko Rakonjac (3), Aleksandar Lučić (2)

iForest - Biogeosciences and Forestry, Volume 16, Issue 6, Pages 307-313 (2023)
doi: https://doi.org/10.3832/ifor4322-016
Published: Nov 09, 2023 - Copyright © 2023 SISEF

Research Articles

In light of the increase of environmental pollution, we tested the effect of cadmium (Cd) and lead (Pb) soil contamination on ash dieback. The experiment included the inoculation of Hymenoscyphus fraxineus on Fraxinus excelsior and Fraxinus angustifolia seedlings growing on unpolluted soil, soil contaminated with cadmium (Cd), and soil contaminated with lead (Pb). At the end of the experiment, 173 days after soil contamination and 50 days since inoculation, all F. excelsior and F. angustifolia seedlings inoculated with H. fraxineus showed ash dieback symptoms in comparison to their control groups. However, both F. excelsior and F. angustifolia seedlings grown on contaminated soil had significantly increased necrotic lesions in comparison to the seedlings grown on uncontaminated soil. Our results showed for the first time that cadmium (Cd) and lead (Pb) soil contamination can significantly contribute to ash dieback and increase damage to F. excelsior and F. angustifolia seedlings.


Soil Contamination, Cadmium (Cd), Lead (Pb), Fraxinus excelsior

Authors’ address

Aleksandar Vemić 0000-0001-9195-8967
Department of Forest Protection, Faculty of Forestry, Kneza Višeslava 1, 11030, Belgrade (Serbia)
Vladan Popović 0000-0003-4326-3364
Aleksandar Lučić 0000-0002-4473-1791
Department of Genetics, Plant Breeding, Seed and Nursery Production; Institute of Forestry, Kneza Višeslava 3, 11030, Belgrade (Serbia)
Zoran Miletić 0000-0001-7416-4647
Ljubinko Rakonjac 0000-0002-8736-6771
Department of Forest Establishment, Silviculture and Ecology, Institute of Forestry, Kneza Višeslava 3, 11030, Belgrade (Serbia)
Zlatan Radulović 0000-0003-2336-0255
Department of Forest Protection, Institute of Forestry, Kneza Višeslava 3, 11030, Belgrade (Serbia)

Corresponding author

Vladan Popović


Vemić A, Popović V, Miletić Z, Radulović Z, Rakonjac L, Lučić A (2023). Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings. iForest 16: 307-313. - doi: 10.3832/ifor4322-016

Academic Editor

Daniela Baldantoni

Paper history

Received: Feb 06, 2023
Accepted: Sep 11, 2023

First online: Nov 09, 2023
Publication Date: Dec 31, 2023
Publication Time: 1.97 months

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