iForest - Biogeosciences and Forestry


Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Vanja Vuksanović (1)   , Branislav Kovačević (2), Srdan Stojnić (2), Marko Kebert (2), Lazar Kesić (2), Vladislava Galović (2), Saša Orlović (1)

iForest - Biogeosciences and Forestry, Volume 15, Issue 4, Pages 265-272 (2022)
doi: https://doi.org/10.3832/ifor4033-015
Published: Jul 25, 2022 - Copyright © 2022 SISEF

Research Articles

The effects of drought simulated via osmotic stress induced by polyethylene glycol (PEG) in the growing medium were examined on two Wild cherry (Prunus avium L.) clones (6A and 8A) based on thirteen morphometric, physiological, and biochemical traits. The shoot tips were exposed to two PEG concentrations (20 and 50 g L-1) in growing medium designed for micropropagation with axillary buds. The results showed that all morphological and physiological traits were significantly reduced, indicating a strong detrimental effect of increased PEG concentrations. The significant decline of radical scavenging activity against ABTS•+ and total content of flavonoids (TFC) and phenols (TPC) were recorded in both clones as a response to high PEG concentrations, whereas opposite trends were noticed for ferric reducing antioxidant power (FRAP). Clone 8A achieved better performance, having more intensive growth, higher multiplication percentage, higher values of carotenoids, more intense decrement of ABTS and increment of FRAP values compared to the clone 6A. The results of the principal component analysis indicate that parameter TFC/TPC in both stem and leaves, as well as TFC in the stems, achieved the strongest relation with morphometric parameters. Our results confirm the feasibility of in vitro evaluation of drought tolerance of Wild cherry, supporting further research on the variability of examined traits in this noble broadleaved tree species.


Drought, Selection, Prunus avium, Tissue Culture, Oxidative Stress

Authors’ address

Vanja Vuksanović 0000-0002-1771-9770
Saša Orlović 0000-0002-2724-1862
University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, 21000 Novi Sad (Serbia)
Branislav Kovačević 0000-0002-9125-0659
Srdan Stojnić
Marko Kebert 0000-0003-0171-6150
Lazar Kesić 0000-0003-2643-9727
Vladislava Galović
University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Cehova 13d, 21000 Novi Sad (Serbia)

Corresponding author



Vuksanović V, Kovačević B, Stojnić S, Kebert M, Kesić L, Galović V, Orlović S (2022). Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro. iForest 15: 265-272. - doi: 10.3832/ifor4033-015

Academic Editor

Federica Brunoni

Paper history

Received: Dec 03, 2021
Accepted: May 11, 2022

First online: Jul 25, 2022
Publication Date: Aug 31, 2022
Publication Time: 2.50 months

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