iForest - Biogeosciences and Forestry


Shifts in the arbuscular mycorrhizal fungal community composition of Betula alnoides along young, middle-aged plantation and adjacent natural forest

Yuebo Jing (1-2), Tao Li (1), Hongliang Cui (3), Lingfei Li (4), C Allen Samuel (5), Lin Chen (1), Yongpeng Li (2), Zhiwei Zhao (1)   

iForest - Biogeosciences and Forestry, Volume 13, Issue 5, Pages 447-455 (2020)
doi: https://doi.org/10.3832/ifor3515-013
Published: Oct 07, 2020 - Copyright © 2020 SISEF

Research Articles

Betula alnoides is a fast-growing and native timber species prevalently planted in tropical and subtropical areas of southern China. Despite the large-scale development of B. alnoides plantations, knowledge of its association with arbuscular mycorrhizal fungi (AMF) is limited. In the present study, we chose young (3-year-old sapling, P3y) and middle-aged (12-year-old stand, P12y) B. alnoides plantations and adjacent native forest (N) in the Puwen Tropical Forest Experimental Station located in Xishuangbanna prefecture of Yunnan Province, southwestern China, as study materials and explored the change in AMF community composition in the plantation chronosequence. In addition, we combined morphological methods and Illumina MiSeq sequencing techniques to analyze rhizosphere soil AMF. The results indicated that the AMF richness and diversity indexes of B. alnoides at two ages tended to be similar to those of natural growing trees in native forest. However, the specific AM fungal compositions were distinctly different, providing evidence of the conservation value of the native forest, which harbors a unique AMF diversity. Hierarchical cluster analysis further revealed that the AMF community composition of trees in the mid-aged stand (P12y) was more similar to that of naturally growing B. alnoides (N) than that of the young-aged trees (P3y), which proved the considerable resilience of AMF to the establishment of the B. alnoides plantation. A set of at least five soil properties (available phosphorus, available nitrogen, organic matter, total nitrogen and silt content) was found to play a significant role in shaping the AMF communities. These results contribute to the understanding of the impacts of B. alnoides plantations on AMF diversity and composition. Such information is critical for the efficient planting and sustainable management of B. alnoides plantations.


Arbuscular Mycorrhizal Fungi, Betula alnoides, Plantation, Native Forest

Authors’ address

Yuebo Jing
Tao Li 0000-0001-8121-0111
Lin Chen
Zhiwei Zhao 0000-0001-9451-9847
Laboratory of Conservation and Utilization for Bioresources and Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming, 650091 Yunnan (China)
Yuebo Jing
Yongpeng Li
Yunnan Academy of Forestry and Grassland, Kunming, 650201 Yunnan (China)
Hongliang Cui
Yunnan Reascend Tobacoo Technology (group) Co., Ltd., Kunming, 650106 Yunnan (China)
Lingfei Li
College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201 Yunnan (China)
C Allen Samuel 0000-0002-3129-5759
Department of Plant and Environmental Sciences, Agricultural Science Center, New Mexico State University, Farmington, NM 87401 (USA)

Corresponding author

Zhiwei Zhao


Jing Y, Li T, Cui H, Li L, Allen Samuel C, Chen L, Li Y, Zhao Z (2020). Shifts in the arbuscular mycorrhizal fungal community composition of Betula alnoides along young, middle-aged plantation and adjacent natural forest. iForest 13: 447-455. - doi: 10.3832/ifor3515-013

Academic Editor

Werther Guidi Nissim

Paper history

Received: May 15, 2020
Accepted: Aug 04, 2020

First online: Oct 07, 2020
Publication Date: Oct 31, 2020
Publication Time: 2.13 months

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