iForest - Biogeosciences and Forestry


Biochar amendment regulated growth, physiological, and biochemical responses of conifer in red soil

Khan Tarin Muhammad Waqqas (1), Lili Fan (2), Yueqin Cai (1), Muhammad Tayyab (3), Lingyan Chen (1), Tianyou He (1), Jundong Rong (2), Yushan Zheng (1-2)   

iForest - Biogeosciences and Forestry, Volume 13, Issue 6, Pages 490-498 (2020)
doi: https://doi.org/10.3832/ifor3416-013
Published: Nov 01, 2020 - Copyright © 2020 SISEF

Research Articles

The addition of Biochar (BC) into the soil is expected to improve soil physicochemical properties and plant growth. However, few studies have verified such an effect on the growth and physiological characteristics of conifers. The current study aims to assess the efficacy of novel physiological parameters as an indicator for assessing the impact of hardwood biochar (BH) on the development of Fokienia hodginsii seedlings to strengthen our understanding of the impacts of the BH on soil to optimize the achievement of BC-based restoration projects. The BH was applied to the soil under four different levels (0, 5, 20, and 80 g Kg-1 of soil) to assess their influence on the leave’s photosynthetic pigments, photosynthesis (Pn), and biochemical traits of F. hodginsii seedlings in four different seasons, and on biomass and soil physicochemical properties at final harvest under greenhouse conditions for one year. In the first two seasons, BH20 and BH80 amended seedlings responded with an improved photosynthetic rate with more production of photosynthetic pigments and biochemical attributes. However, none of the BC doses increased the Pn of seedlings in the final season. Nonetheless, after one year a rise in soil pH as well as P and K availability resulted in a maximum 25% increase in biomass of F. hodginsii under BH80 amendments. Our findings reveal that the incorporation of BH (20 and 80 g kg-1 of soil) has a substantial positive effect on seedling biomass and soil fertility. However, the application of BH into acidic soils may be effective in restoring degraded soils if initially combined with fertilizers. We recommend a careful approach to the selection of BC because its influence may vary between different soil types, plant species, and BC feedstocks.


Fokienia hodginsii, Hardwood Biochar, Restoration, Photosynthesis

Authors’ address

Khan Tarin Muhammad Waqqas 0000-0001-7636-3893
Yueqin Cai 0000-0002-0684-9342
Lingyan Chen
Tianyou He
Yushan Zheng 0000-0001-9545-0984
College of Arts & College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002 (P.R. China)
Lili Fan
Jundong Rong 0000-0002-8733-8500
Yushan Zheng 0000-0001-9545-0984
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 (P.R. China)
Muhammad Tayyab
College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 (P.R. China)

Corresponding author

Yushan Zheng


Tarin Muhammad Waqqas K, Fan L, Cai Y, Tayyab M, Chen L, He T, Rong J, Zheng Y (2020). Biochar amendment regulated growth, physiological, and biochemical responses of conifer in red soil. iForest 13: 490-498. - doi: 10.3832/ifor3416-013

Academic Editor

Daniela Baldantoni

Paper history

Received: Mar 31, 2020
Accepted: Aug 15, 2020

First online: Nov 01, 2020
Publication Date: Dec 31, 2020
Publication Time: 2.60 months

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Abideen Z, Koyro H-W, Huchzermeyer B, Bilquees GUL, Khan MA (2020)
Impact of a biochar or a biochar-compost mixture on water relation, nutrient uptake and photosynthesis of Phragmites karka. Pedosphere 30 (4): 466-477.
CrossRef | Gscholar
Agegnehu G, Bass AM, Nelson PN, Muirhead B, Wright G, Bird MI (2015)
Biochar and biochar-compost as soil amendments: effects on peanut yield, soil properties and greenhouse gas emissions in tropical North Queensland, Australia. Agriculture, Ecosystems and Environment 213: 72-85.
CrossRef | Gscholar
Anyanwu IN, Alo MN, Onyekwere AM, Crosse JD, Nworie O, Chamba EB (2018)
Influence of biochar aged in acidic soil on ecosystem engineers and two tropical agricultural plants. Ecotoxicology and Environmental Safety 153: 116-126.
CrossRef | Gscholar
Backer RGM, Saeed W, Seguin P, Smith DL (2017)
Root traits and nitrogen fertilizer recovery efficiency of corn grown in biochar-amended soil under greenhouse conditions. Plant and Soil 415: 1-2.): 465-477.
CrossRef | Gscholar
Baronti S, Vaccari FP, Miglietta F, Calzolari C, Lugato E, Orlandini S, Pini R, Zulian C, Genesio L (2014)
Impact of biochar application on plant water relations in Vitis vinifera (L.). European Journal of Agronomy 53: 38-44.
CrossRef | Gscholar
Biederman LA, Phelps J, Ross BJ, Polzin M, Harpole WS (2017)
Biochar and manure alter few aspects of prairie development: a field test. Agriculture, Ecosystems and Environment 236: 78-87.
CrossRef | Gscholar
Bradford MM (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
CrossRef | Gscholar
Bu X, Xue J, Wu Y, Ma W (2020)
Effect of biochar on seed germination and seedling growth of Robinia pseudoacacia L. in karst calcareous soils. Communications in Soil Science and Plant Analysis 51 (3): 352-363.
CrossRef | Gscholar
Cai YQ, Tarin MWK, Fan LL, Xie DJ, Rong JD, He TY, Chen LG, Zheng YS (2020)
Responses of photosynthesis, chloroplast ultrastructure, and antioxidant system of Morinda officinalis how. to exogenous 2.4-epibrassinolide treatments under high temperature stress. Applied Ecology and Environmental Research 18: 3981-4004.
CrossRef | Gscholar
Cakmak I, Horst WJ (1991)
Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum 83 (3): 463-468.
CrossRef | Gscholar
Chen L, Lai J, He T, Rong J, Tarin MWK, Zheng Y (2018)
Differences in photosynthesis of variegated temple bamboo leaves with various levels of variegation are related to chlorophyll biosynthesis and chloroplast development. Journal of the American Society for Horticultural Science 143 (2): 144-153.
CrossRef | Gscholar
Choi D, Makoto K, Quoreshi AM, Qu L (2009)
Seed germination and seedling physiology of Larix kaempferi and Pinus densiflora in seedbeds with charcoal and elevated CO2. Landscape and Ecological Engineering 5 (2): 107-113.
CrossRef | Gscholar
Choi HS, Zhao Y, Dou H, Cai X, Gu M, Yu F (2018)
Effects of biochar mixtures with pine-bark based substrates on growth and development of horticultural crops. Horticulture Environment and Biotechnology 59 (3): 345-354.
CrossRef | Gscholar
De Assis Gomes MDM, Magalhães Andrade Lagôa AM, Medina CL, Machado EC, Machado MA (2004)
Interactions between leaf water potential, stomatal conductance and abscisic acid content of orange trees submitted to drought stress. Brazilian Journal of Plant Physiology 16 (3): 155-161.
CrossRef | Gscholar
De Carvalho Goncalves JF, De Sousa Barreto DC, Dos Santos UM, Fernandes AV, Barbosa Sampaio PDT, Buckeridge MS (2005)
Growth, photosynthesis and stress indicators in young rosewood plants (Aniba rosaeodora Ducke) under different light intensities. Brazilian Journal of Plant Physiology 17: 325-334.
CrossRef | Gscholar
Du Z, Xiao Y, Qi X, Liu Y, Fan X, Li Z (2018)
Peanut-shell biochar and biogas slurry improve soil properties in the North China plain: a four-year field study. Scientific Reports 8 (1): 1-9.
CrossRef | Gscholar
Farquhar GD, Sharkey TD (1982)
Stomatal conductance and photosynthesis. Annual Review of Plant Physiology 33 (1): 317-345.
CrossRef | Gscholar
Gezelius K, Hallen M (1980)
Seasonal variation in ribulose bisphosphate carboxylase activity in Pinus silvestris. Physiologia Plantarum 48 (1): 88-98.
CrossRef | Gscholar
Haefele SM, Konboon Y, Wongboon W, Amarante S, Maarifat AA, Pfeiffer EM, Knoblauch C (2011)
Effects and fate of biochar from rice residues in rice-based systems. Field Crops Research 121 (3): 430-440.
CrossRef | Gscholar
Haynes RJ, Mokolobate MS (2001)
Amelioration of Al toxicity and P deficiency in acid soils by additions of organic residues: a critical review of the phenomenon and the mechanisms involved. Nutrient Cycling in Agroecosystems 59 (1): 47-63.
CrossRef | Gscholar
Kammann CI, Linsel S, Göling JW, Koyro H-W (2011)
Influence of biochar on drought tolerance of Chenopodium quinoa Willd and on soil-plant relations. Plant and Soil 345 (1-2): 195-210.
CrossRef | Gscholar
Kazemi N, Khavari-Nejad RA, Fahimi H, Saadatmand S, Nejad-Sattari T (2010)
Effects of exogenous salicylic acid and nitric oxide on lipid peroxidation and antioxidant enzyme activities in leaves of Brassica napus L. under nickel stress. Scientia Horticulturae 126 (3): 402-407.
CrossRef | Gscholar
Keller T, Schwager H (1977)
Air pollution and ascorbic acid. European Journal of Forest Pathology 7 (6): 338-350.
CrossRef | Gscholar
Levesque V, Rochette P, Ziadi N, Dorais M, Antoun H (2018)
Mitigation of CO2, CH4 and N2O from a fertigated horticultural growing medium amended with biochars and a compost. Applied Soil Ecology 126: 129-139.
CrossRef | Gscholar
Lichtenthaler HK (1987)
Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: “Methods in Enzymology”. Elsevier, Amsterdam, Netherlands, vol. 148, pp. 350-382.
CrossRef | Gscholar
Lundmark T, Hedén J, Hällgren J-E (1988)
Recovery from winter depression of photosynthesis in pine and spruce. Trees 2 (2): 110-114.
CrossRef | Gscholar
Lusiba S, Odhiambo J, Ogola J (2018)
Growth, yield and water use efficiency of chickpea (Cicer arietinum): response to biochar and phosphorus fertilizer application. Archives of Agronomy and Soil Science 64 (6): 819-833.
CrossRef | Gscholar
Makoto K, Hirobe M, DeLuca TH, Bryanin SV, Procopchuk VF, Koike T (2011)
Effects of fire-derived charcoal on soil properties and seedling regeneration in a recently burned Larix gmelinii/Pinus sylvestris forest. Journal of Soils and Sediments 11 (8): 1317-1322.
CrossRef | Gscholar
Mansfield TA, Hetherington AM, Atkinson CJ (1990)
Some current aspects of stomatal physiology. Annual Review of Plant Biology 41 (1): 55-75.
CrossRef | Gscholar
Mastalerczuk G, Borawska-Jarmulowicz B, Kalaji HM, Dabrowski P, Paderewski J (2017)
Gas-exchange parameters and morphological features of festulolium (Festulolium braunii K. Richert A. Camus) in response to nitrogen dosage. Photosynthetica 55 (1): 20-30.
CrossRef | Gscholar
Naidu CV, Swamy PM (1995)
Seasonal variation in ribulose 1.5-bisphosphate carboxylase activity and its relationship with leaf protein content and net photosynthetic rate in tropical deciduous tree species. Photosynthetica 31 (1): 85-90.
Naz H, Akram NA, Ashraf M (2016)
Impact of ascorbic acid on growth and some physiological attributes of cucumber (Cucumis sativus) plants under water-deficit conditions. Pakistan Journal of Botany 48 (3): 877-883.
Online | Gscholar
Nobile C, Denier J, Houben D (2019)
Linking biochar properties to biomass of basil, lettuce and pansy cultivated in growing media. Scientia Horticulturae. 261: 109001.
CrossRef | Gscholar
Omil B, Pineiro V, Merino A (2013)
Soil and tree responses to the application of wood ash containing charcoal in two soils with contrasting properties. Forest Ecology and Management 295: 199-212.
CrossRef | Gscholar
Paneque M, Jose M, Franco-Navarro JD, Colmenero-Flores JM, Knicker H (2016)
Effect of biochar amendment on morphology, productivity and water relations of sunflower plants under non-irrigation conditions. Catena 147: 280-287.
CrossRef | Gscholar
Pansu M, Gautheyrou J (2007)
Handbook of soil analysis: mineralogical, organic and inorganic methods. Springer Science and Business Media, Berlin-Heidelberg-New York, pp. 993.
Online | Gscholar
Pluchon N, Gundale MJ, Nilsson M, Kardol P, Wardle DA (2014)
Stimulation of boreal tree seedling growth by wood-derived charcoal: effects of charcoal properties, seedling species and soil fertility. Functional Ecology 28 (3): 766-775.
CrossRef | Gscholar
Prendergast-Miller MT, Duvall M, Sohi SP (2014)
Biochar-root interactions are mediated by biochar nutrient content and impacts on soil nutrient availability. European Journal of Soil Science 65 (1): 173-185.
CrossRef | Gscholar
Sarauer JL, Coleman MD (2018)
Biochar as a growing media component for containerized production of Douglas-fir. Canadian Journal of Forest Research 48 (5): 581-588.
CrossRef | Gscholar
Shaaban M, Van Zwieten L, Bashir S, Younas A, Núñez-Delgado A, Chhajro MA, Kubar KA, Ali U, Rana MS, Mehmood MA (2018)
A concise review of biochar application to agricultural soils to improve soil conditions and fight pollution. Journal of Environmental Management 228: 429-440.
CrossRef | Gscholar
Shao H-B, Chu L-Y, Jaleel CA, Zhao C-X (2008)
Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies 331 (3): 215-225.
CrossRef | Gscholar
Sheu BH, Lin CK (1999)
Photosynthetic response of seedlings of the sub-tropical tree Schima superba with exposure to elevated carbon dioxide and temperature. Environmental and Experimental Botany 41 (1): 57-65.
CrossRef | Gscholar
Streb P, Shang W, Feierabend J, Bligny R (1998)
Divergent strategies of photoprotection in high-mountain plants. Planta 207 (2): 313-324.
CrossRef | Gscholar
Tarin MWK, Lili F, Lu S, Jinli L, Jingwen L, Zhiwen D, Lingyan C, Tianyou H, Rong J, Zheng Y (2020)
Rice straw biochar impact on physiological and biochemical attributes of Fokienia hodginsii in acidic soil. Scandinavian Journal of Forest Research 35 (1-2): 59-68.
CrossRef | Gscholar
Thomas SC, Frye S, Gale N, Garmon M, Launchbury R, Machado N, Melamed S, Murray J, Petroff A, Winsborough C (2013)
Biochar mitigates negative effects of salt additions on two herbaceous plant species. Journal of Environmental Management 129: 62-68.
CrossRef | Gscholar
Uzoma KC, Inoue M, Andry H, Fujimaki H, Zahoor A, Nishihara E (2011)
Effect of cow manure biochar on maize productivity under sandy soil condition. Soil Use and Management 27 (2): 205-212.
CrossRef | Gscholar
Videgain-Marco M, Marco-Montori P, Martí-Dalmau C, Jaizme-Vega MC, Manyà-Cervelló JJ, García-Ramos FJ (2020)
Effects of biochar application in a sorghum crop under greenhouse conditions: growth parameters and physicochemical fertility. Agronomy 10 (1): 104.
CrossRef | Gscholar
Wang Y, Pan F, Wang G, Zhang G, Wang Y, Chen X, Mao Z (2014)
Effects of biochar on photosynthesis and antioxidative system of Malus hupehensis Rehd. seedlings under replant conditions. Scientia Horticulturae 175: 9-15.
CrossRef | Gscholar
Watanabe FS, Olsen SR (1965)
Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Science Society of America Journal 29 (6): 677-678.
CrossRef | Gscholar
Weng JH, Liao TS, Sun KH, Chung JC, Lin CP, Chu CH (2005)
Seasonal variations in photosynthesis of Picea morrisonicola growing in the subalpine region of subtropical Taiwan. Tree Physiology 25 (8): 973-979.
CrossRef | Gscholar
Xu CY, Hosseini-Bai S, Hao Y, Rachaputi RCN, Wang H, Xu Z, Wallace H (2015)
Effect of biochar amendment on yield and photosynthesis of peanut on two types of soils. Environmental Science and Pollution Research 22 (8): 6112-6125.
CrossRef | Gscholar
Yuanyuan M, Yali Z, Jiang L, Hongbo S (2010)
Roles of plant soluble sugars and their responses to plant cold stress. African Journal of Biotechnology 8 (10): 2004-2010.
Online | Gscholar
Zahedifar M, Najafian S (2017)
Ocimum basilicum L. growth and nutrient status as influenced by biochar and potassium-nano chelate fertilizers. Archives of Agronomy and Soil Science 63 (5): 638-650.
CrossRef | Gscholar
Zemanova V, Brendova K, Pavlikova D, Kubatova P, Tlustos P (2017)
Effect of biochar application on the content of nutrients (Ca, Fe, K, Mg, Na, P) and amino acids in subsequently growing spinach and mustard. Plant, Soil and Environment 63 (7): 322-327.
CrossRef | Gscholar

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