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Leaf morphology of progenies in Q. suber, Q. ilex, and their hybrids using multivariate and geometric morphometric analysis

Unai López De Heredia   , María José Duro-García, Álvaro Soto

iForest - Biogeosciences and Forestry, Volume 11, Issue 1, Pages 90-98 (2018)
doi: https://doi.org/10.3832/ifor2577-010
Published: Jan 31, 2018 - Copyright © 2018 SISEF

Research Articles

Collection/Special Issue: INCOTW - Sassari, Italy (2017)
International Congress on Cork Oak Trees and Woodlands
Guest Editors: Piermaria Corona, Sandro Dettori


The genus Quercus is known for the occurrence of frequent hybridization events between species. Although this phenomenon is not common among holm oak (Q. ilex) and cork oak (Q. suber), these species can hybridize when they coexist in mixed stands. The result of hybridization is a viable hybrid progeny with very heterogeneous leaf morphology. Literature concerning the leaf morphology of suber-ilex hybrid seedlings is scarce, and non-existent from a quantitative point of view. In the case of the leaf morphology of hybrids and their progeny, it has been observed a high frequency of leaves with fluctuating asymmetry or developmental abnormalities, which can have a marked effect on fitness. In this work, we have characterized seedlings’ leaf morphology corresponding to two- and four-year-old half-sib progenies of holm oak, cork oak and their hybrids. For this purpose, three to ten leaves of each individual were collected, and two methodologies were used for analysis. Firstly, we used a classic morphological analysis of twelve variables that were reduced using multivariate techniques. On the other hand, shape of the leaves was thoroughly analyzed by geometric morphometric analysis methods. The extent of fluctuating asymmetry and the presence of developmental abnormalities of seedlings were analyzed calculating an asymmetry index. The results indicate that thickness is the most discriminating trait between species, and that the hybrid progenies do not show a third different phenotype compared to the parental species. However, half-siblings tend to show similar leaf morphology between them, depending on the genetic adscription of the parents. While fluctuating asymmetry was found in half-sib progenies of the parental species and the hybrids, a significant proportion of hybrid half-sibs showed strong leaf asymmetry, probably due to modifications of the epigenetic systems that control leaf development at the shoot apical meristems and leaf primordia.

  Keywords


Hybridization, Fluctuating Asymmetry, Leaf Morphology, Procrustes Analysis

Authors’ address

(1)
Unai López De Heredia
María José Duro-García
Álvaro Soto
GI Genética, Fisiología e Historia Forestal, Dept. Sistemas y Recursos Naturales, ETSI Montes, Forestal y del Medio Natural, Universidad Politécnica de Madrid (Spain)

Corresponding author

 
Unai López De Heredia
unai.lopezdeheredia@upm.es

Citation

López De Heredia U, Duro-García MJ, Soto Á (2018). Leaf morphology of progenies in Q. suber, Q. ilex, and their hybrids using multivariate and geometric morphometric analysis. iForest 11: 90-98. - doi: 10.3832/ifor2577-010

Academic Editor

Piermaria Corona

Paper history

Received: Jul 31, 2017
Accepted: Oct 28, 2017

First online: Jan 31, 2018
Publication Date: Feb 28, 2018
Publication Time: 3.17 months

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