iForest - Biogeosciences and Forestry


Characterization of two poplar homologs of the GRAS/SCL gene, which encodes a transcription factor putatively associated with salt tolerance

Vladislava Galovic (1)   , Sasa Orlovic (1), Matthias Fladung (2)

iForest - Biogeosciences and Forestry, Volume 8, Issue 6, Pages 780-785 (2015)
doi: https://doi.org/10.3832/ifor1330-008
Published: May 19, 2015 - Copyright © 2015 SISEF

Research Articles

Collection/Special Issue: COST Action FP0905
Biosafety of forest transgenic trees and EU policy directives
Guest Editors: Cristina Vettori, Matthias Fladung

To cope with soil salinity, which is one of the most severe forms of abiotic stress, efforts are being undertaken to enhance the salt tolerance of economically important poplar clones in the Vojvodina region of Serbia. One approach is to screen nucleotide diversity in candidate genes (CG) in several poplar clones of high economic importance to Serbia to search for associations with salt stress tolerance. As plant-specific GRAS/SCL transcription factors (TFs) play diverse roles in abiotic stress resistance, two poplar homologs of GRAS/SCL TFs were chosen to differentiate the species background with respect to salt tolerance. A BLAST search of the Populus trichocarpa genome using the P. euphratica gene GRAS/SCL TF_GH611858 sequence identified two putative orthologs, Scaf_5 and Scaf_7, with identities of 100% and 94%, respectively. Primers were designed in identical sequences of Scaf_5 and Scaf_7 to amplify fragments of GRAS/SCL TF orthologs in four poplar clones that are economically important to Serbia. The primers spanned regions where, at least in P. trichocarpa, single nucleotide polymorphisms (SNPs) are present, thereby increasing the probability of distinguishing Scaf_5 and Scaf_7 orthologs in the four clones. Alignments and analyses of the gene fragments revealed that both orthologs were representative of the genetic diversity between different poplar clones, and the identified SNP markers differentiated the four poplar clones with respect to salt tolerance.


Candidate Gene, Nucleotide Polymorphism, Tree Genomics, Poplar

Authors’ address

Vladislava Galovic
Sasa Orlovic
University of Novi Sad, Institute of Lowland, Forestry and Environment, Antona Cehova 13, 21000 Novi Sad (Serbia)
Matthias Fladung
Thuenen Institute of Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf (Germany)

Corresponding author

Vladislava Galovic


Galovic V, Orlovic S, Fladung M (2015). Characterization of two poplar homologs of the GRAS/SCL gene, which encodes a transcription factor putatively associated with salt tolerance. iForest 8: 780-785. - doi: 10.3832/ifor1330-008

Academic Editor

Marco Borghetti

Paper history

Received: Apr 29, 2014
Accepted: Feb 12, 2015

First online: May 19, 2015
Publication Date: Dec 01, 2015
Publication Time: 3.20 months

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List of the papers citing this article based on CrossRef Cited-by.

Bolle C, Koncz C, Chua NH (2000)
PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction. Genes and Development 14: 1269-1278.
Online | Gscholar
Bolle C (2004)
The role of GRAS proteins in plant signal transduction and development. Planta 218: 683-692.
CrossRef | Gscholar
Cervera MT, Storme V, Soto A, Ivens B, Van Montagu M, Rajora OP, Boerjan W (2005)
Intraspecific and interspecific genetic and phylogenetic relationships in the genus Populus based on AFLP markers. Theoretical and Applied Genetics 111: 1440-1456.
CrossRef | Gscholar
Chen S, Polle A (2010)
Salinity tolerance of Populus. Plant Biology 12: 317-333.
CrossRef | Gscholar
Dayanandan S, Rajora OP, Bawa KS (1998)
Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides). Theoretical and Applied Genetics 96: 950-956.
CrossRef | Gscholar
Dvornyk V, Vinogradova O, Eviatar N (2002)
Long-term microclimatic stress causes rapid adaptive radiation of kaiABC clock gene family in a cyanobacterium, Nostoc linckia, from “Evolution Canyons” I and II, Israel. Proceedings of the National Academy of Science USA 99: 2082-2087.
CrossRef | Gscholar
Fladung M, Buschbom J (2009)
Identification of single nucleotide polymorphisms in different Populus species. Trees 23: 1199-1212.
CrossRef | Gscholar
Galovic V, Mladenovic-Drinic S, Jelovac D, Navalusic J (2004)
Application possibilities of AFLP fingerprinting technique in maize DNA profiling and plant variety protection. Genetika 36: 133-142.
CrossRef | Gscholar
Galovic V, Orlovic S (2007)
Overview of DNA-based studies of genetic variability in poplars. Topola 179/180: 71-79. [in Serbian with English summary]
Gu R, Fonseca S, Puskas LG, Hackler L, Zvara Á, Dudits D, Pais MS (2004)
Transcript identification and profiling during salt stress and recovery of Populus euphratica. Tree Physiology 24: 265-276.
CrossRef | Gscholar
Ivanišević P, Galić Z, Rončević S, Kovačević B, Marković M (2008)
Significance of establishment of forest tree and shrub plantations for the stability and sustainable development of ecosystems in Vojvodina. Topola 181/182: 31-41. [in Serbian with English summary]
Ma HS, Liang D, Shuai P, Xia XL, Yin WL (2010)
The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana. Journal of Experimental Botany 61: 4011-4019.
CrossRef | Gscholar
Orlovic S, Klasnja B, Ivanisevic P (1997)
Anatomical and physiological research in a poplar breeding program. Topola 159/160: 69-80. [in Serbian with English summary]
Orlovic S, Galovic V, Zoric M, Kovacevic B, Pilipovic A, Galic Z (2009)
Evaluation of interspecific DNA variability in poplars using AFLP and SSR markers. African Journal of Biotechnology 8: 5241-5247.
Online | Gscholar
Physh LD, Wysocka-Diller JW, Camilleri C, Bouchez D, Benfey PN (1999)
The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes. Plant Journal 18: 111-119.
CrossRef | Gscholar
Rahman MH, Dayanandan S, Rajora OP (2000)
Microsatellite DNA markers in Populus tremuloides. Genome 43: 293-297.
CrossRef | Gscholar
Rahman MH, Rajora OP (2002)
Microsatellite DNA fingerprinting, differentiation and genetic relationships of clones, cultivars and varieties of six poplar species from three sections of the genus Populus. Genome 45: 1083-1094.
CrossRef | Gscholar
Rajora OP, Rahman MH (2003)
Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus × canadensis) cultivars. Theoretical and Applied Genetics 106: 470-477.
Online | Gscholar
Sairam RK, Tyagi A (2004)
Physiology and molecular biology of salinity stress tolerance in plants. Current Science 86: 407-421.
Online | Gscholar
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Dejardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjarvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leple JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouze P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D (2006)
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313 (5793): 1596-1604.
CrossRef | Gscholar
Van der Schoot J, Popiskova M, Vosman B, Smulders MJM (2000)
Development and characterization of microsatellite markers in black poplar (Populus nigra L.). Theoretical and Applied Genetics 101: 317-322.
CrossRef | Gscholar
Zsuffa L (1975)
A summary review of interspecific breeding in the genus Populus L. In: Proceedings of the “14th Meeting of the Canadian Tree Improvement Association” Part 2. Fredericton (NB, Canada). Canadian Forest Service, Ottava, ON, Canada, pp. 107-123.

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