For most plants transformation methods and regeneration protocols are readily available, leading to more complicated and differential research in plant science. The application of expression or RNAi constructs in biotechnology can lead to unwanted effects and detrimental phenotypes, which may cause great problems in the regeneration and breeding of transgenic plants. To circumvent this set back we resort to the transformation of inactive constructs. After the creation and establishment of transgenic lines the construct is specifically activated and the effects on the plants can be studied in detail, even if the resulting phenotype has harmful consequences. The activation of the constructs is carried out by means of induced site-directed recombination. The application of an heat shock leads to the expression of a recombinase, which excises the spacer fragment and leads to the desired effect on the plants. The feasibility of those constructs could be shown for RNAi, induced expression and even marker excision and confinement approaches demonstrating the huge variability of the new construct design.
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Citation
Hinze A, Becker D (2012). Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands. iForest 5: 131-137. - doi: 10.3832/ifor0620-005
Academic Editor
Gabriele Bucci
Paper history
Received: Jan 24, 2012
Accepted: May 23, 2012
First online: Jun 13, 2012
Publication Date: Jun 29, 2012
Publication Time: 0.70 months
© SISEF - The Italian Society of Silviculture and Forest Ecology 2012
Open Access
This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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