iForest - Biogeosciences and Forestry


Mapping Cadmium distribution in roots of Salicaceae through scanning electron microscopy with x-ray microanalysis

C Cocozza (1)   , L Maiuro (2), R Tognetti (1)

iForest - Biogeosciences and Forestry, Volume 4, Issue 3, Pages 113-120 (2011)
doi: https://doi.org/10.3832/ifor0563-004
Published: Jun 01, 2011 - Copyright © 2011 SISEF

Research Articles

A screening in hydroponics was carried out to assess the potential of Cd uptake and metal accumulation in roots of 4 poplar and 2 willow clones. Rooted cuttings were exposed for 3 weeks to 50 μM Cd sulphate in a growth chamber. Salicaceae clones were discriminated in their potential to absorb and distribute Cd in root tissues considering the interaction between metal and macronutrients. SEM-EDXMA was useful to investigate structural modifications and to identify in situ concentration and distribution of elements in roots, establishing indirect correlations between accumulation and localization of the metal. Overall, observations suggested differential patterns between species and clones in accumulating Cd within the root profile. In conclusion, Cd accumulation in root levels would hardly be a useful indicator of the ability of plants to translocate metal, independently from other parameters, while Cd location in the root cross-section levels could suggest clone-specific control mechanism to mineral uptake.


Cadmium, EDXMA-SEM, Salicaceae clones, Phytoremediation, Roots

Authors’ address

C Cocozza
R Tognetti
Dipartimento di Scienze e Tecnologie per l’Ambiente e il Territorio (STAT), Università del Molise, c.da Fonte Lappone, I-86090 Pesche (Italy)
L Maiuro
Dipartimento di Scienze e Tecnologie Agro-Alimentari Ambientali e Microbiologiche, Università del Molise, v. De Sanctis, I-86100 Campobasso (Italy)

Corresponding author


Cocozza C, Maiuro L, Tognetti R (2011). Mapping Cadmium distribution in roots of Salicaceae through scanning electron microscopy with x-ray microanalysis. iForest 4: 113-120. - doi: 10.3832/ifor0563-004

Paper history

Received: Oct 05, 2010
Accepted: Mar 01, 2011

First online: Jun 01, 2011
Publication Date: Jun 01, 2011
Publication Time: 3.07 months

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List of the papers citing this article based on CrossRef Cited-by.

Alcántara E, Romera FJ, Cañete M, de La Guardia MD (1994)
Effects of heavy metals on both induction and function of root Fe (III) reductase in Fe-deficient cucumber (Cucumis sativus L.) plants. Journal of Experimental Botany 45: 1893-1898.
CrossRef | Gscholar
Arnon DI, Hoagland DR (1940)
Crop production in artificial culture solutions and in soils with special reference to factors influencing yields and absorption of inorganic nutrient. Soil Science 50: 463-483.
Boot RGA, Mensink M (1990)
Size and morphology of root systems of perennial grasses from contrasting habitats as affected by nitrogen supply. Plant and Soil 129: 291-299.
CrossRef | Gscholar
Boulila Zoghlami L, Djebali W, Chaïbi W, Ghorbel MH (2006)
Modifications physiologiques et structurales induites par l’interaction cadmium-calcium chez la tomate (Lycopersicon esculentum). C.R. Biologies 329: 702-711.
CrossRef | Gscholar
Castiglione S, Todeschini V, Franchin C, Torrigiani P, Gastaldi D, Cicatelli A, Rinaudo C, Berta G, Biondi S, Lingua G (2009)
Clonal differences in survival capacity, copper and zinc accumulation, and correlation with leaf polyamine levels in poplar: A large-scale field trial on heavily polluted soil. Environmental Pollution 157: 2108-2117.
CrossRef | Gscholar
Clemens S (2006)
Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie 88: 1707-19.
CrossRef | Gscholar
Cocozza C, Minnocci A, Tognetti R, Iori V, Zacchini M, Scarascia Mugnozza G (2008)
Distribution and concentration of cadmium in root tissue of Populus alba determined by scanning electron microscopy and energy-dispersive x-ray microanalysis. iForest 1: 96-103.
CrossRef | Gscholar
Cohen CK, Fox TC, Garvin DF, Kochian LV (1998)
The role of iron-deficiency stress responses in stimulating heavy-metal transport in plants. Plant Physiology 116: 1063-1072.
CrossRef | Gscholar
Connolly EL, Fett JP, Guerinot ML (2002)
Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation. Plant Cell 14:1347-57.
CrossRef | Gscholar
Deng H, Ye ZH, Wong MH (2004)
Accumulation of lead, zinc, copper and cadmium by 12 wetland plant species thriving in metal-contaminated sites in China. Environmental Pollution 132: 29-40.
CrossRef | Gscholar
Dickinson NM (2000)
Strategies for sustainable woodland on contaminated soils. Chemosphere 41: 259-263.
CrossRef | Gscholar
Dos Santos Utmazian MN, Wieshammer G, Vega R, Wenzel WW (2007)
Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars. Environ Pollution 148: 155-65.
CrossRef | Gscholar
Duncan WG, Ohlrogge AJ (1958)
Principles of nutrient uptake from fertilizer bands. II. Root development in the band. Agronomy Journal 50: 605-608.
Eckert M, Biela A, Siefritz F, Kaldenhoff R (1999)
New aspects of plant aquaporin regulation and specificity. Journal of Experiment Botany 50: 1541-1545.
CrossRef | Gscholar
Fodor F, Gáspár L, Morales F, Gogorcena Y, Lucena JJ, Cseh E, Kröpf K, Abadía J, Sárvári É (2005)
Effects of two iron sources on iron and cadmium allocation in poplar (Populus alba) plants exposed to cadmium. Tree Physiology 25: 1173-1180.
CrossRef | Gscholar
Frey B, Brunner I, Walther P, Scheidegger C, Zierold C (1997)
Element localization in ultrathin cryosections of high-pressure frozen ectomycorrhizal spruce roots. Plant Cell and Environment 20: 929-937
CrossRef | Gscholar
Frey B, Keller C, Zierold K, Schulin R (2000)
Distribution of Zn in functionally different leaf epidermal cells of the hyperaccumulator Thlaspi caerulescens. Plant, Cell and Environment 23: 675-687.
CrossRef | Gscholar
Haynes RJ (1980)
Ion exchange properties of roots and ionic interactions within the root apoplasm: their role in ion accumulation by plants. Botanical Review 46: 75-99.
CrossRef | Gscholar
Hinkle PM, Shanshala ED, Nelson EJ (1992)
Measurement of intracellular cadmium with fluorescent dyes. Further evidence for the role of calcium channels in cadmium uptake. Journal of Biological Chemistry 267: 25553-25557.
Kieffer P, Schröder P, Dommes J, Hoffmann L, Renaut J, Hausman JF (2009a)
Proteomic and enzymatic response of poplar to cadmium stress. Journal of Proteomics 72: 379 - 396.
CrossRef | Gscholar
Kieffer P, Planchon S, Oufir M, Ziebel J, Dommes J, Hoffmann L, Hausman JF, Renaut J (2009b)
Combining proteomics and metabolite analyses to unravel cadmium stress-response in poplar leaves. Journal of Proteome Research 9: 400-17.
CrossRef | Gscholar
Lasat MM, Fuhrmann M, Ebbs SD, Cornish JE, Kochian LV (1998)
Phytoremediation of a radiocesium contaminated soil: evaluation of cesium- 137 bioaccumulation in the shoots of three plant species. Journal of Environmental Quality 27: 165-168.
CrossRef | Gscholar
Lasat MM, Norvell WA, Kochian LV (1997)
Potential for phytoextraction of 137Cs from a contaminated soil. Plant Soil 195: 99-106.
CrossRef | Gscholar
Laureysens I, De Temmerman L, Hastir T, Van Gysel M, Ceulemans R (2005)
Clonal variation in heavy metal accumulation and biomass production in a poplar coppice culture. II. Vertical distribution and phytoextraction potential. Environmental Pollution 133: 541-551.
CrossRef | Gscholar
Lunácková L, A Sottníková A, Masarovicová E, Lux A, Streško V (2003)
Comparison of cadmium effect on willow and poplar in response to different cultivation conditions. Biologia Plantarum 47: 403 - 411.
CrossRef | Gscholar
Maser P, Thomine S, Schroeder JI, Ward JM,Hirschi K, SzeH, et al. (2001)
Phylogenetic relationships within cation transporter families of Arabidopsis. Plant Physiology 126: 1646-1667.
CrossRef | Gscholar
Merkl N, Schultze-Kraft R, Infante C (2005)
Phytoremediation in the tropics influence of heavy crude oil on root morphological characteristics of graminoids. Environmental Pollution 138: 86-91.
CrossRef | Gscholar
Merrington G, Alloway BJ (1994)
The flux of Cd, Cu, Pb and Zn in mining polluted soils. Water Air Soil and Pollution 73: 333- 344.
CrossRef | Gscholar
Perfus-Barbeoch L, Leonhardt N, Vavasseur A, Forestier C (2002)
Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. Plant Journal 32: 539-548.
CrossRef | Gscholar
Pietrini F, Zacchini M, Iori V, Pietrosanti L, Bianconi D, Massacci A (2010)
Screening of poplar clones for cadmium phytoremediation using photosynthesis, biomass and cadmium content analyses. International Journal of Phytoremediation 12:105-120.
CrossRef | Gscholar
Pulford ID, Watson C (2003)
Phytoremediation of heavy metal-contaminated land by trees - a review. Environment International 29: 529-540.
CrossRef | Gscholar
Robinson BH, Mills TM, Petit D, Fung LE, Green SR, Clothier BE (2000)
Natural and induced cadmium-accumulation in poplar and willow: Implications for phytoremediation. Plant and Soil 227: 301-306.
CrossRef | Gscholar
Salt DE, Smith RD, Raskin I (1998)
Phytoremediation. Annual Review Plant Physiology and Plant Molecular Biology 49: 643-68.
CrossRef | Gscholar
Shen ZG, Zhao FJ, McGrath SP (1997)
Uptake and transport of zinc in the hyperaccumulator Thlaspi caerulescens and the non hyperaccumulator Thlaspi ochroleucum. Plant Cell Environment 20: 898-906.
CrossRef | Gscholar
Soukup A, Armstrong W, Schreiber L, Franke R, Votrubova O (2007)
Apoplastic barriers to radial oxygen loss and solute penetration: a chemical and functional comparison of the exodermis of two wetland species, Phragmites australis and Glyceria maxima. New Phytologist 173: 264-278.
CrossRef | Gscholar
Vert G, Grotz N, Dédaldéchamp F, Gaymard F, Guerinot ML, Briat JF, Curie C (2002)
IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth. The Plant Cell 14: 1223-1233.
CrossRef | Gscholar
Wei S, Zhou Q (2006)
Phytoremediation of cadmium-contaminated soils by Rorippa globosa using two-phase planting. Environmental Science and Pollution Research 13: 151-155.
CrossRef | Gscholar
Williams CH, David DJ (1977)
Some effects of the distribution of cadmium and phosphate in the root zone on the cadmium content of plants. Australian Journal of Soil Research 15: 59-68.
CrossRef | Gscholar
Zacchini M, Pietrini F, Scarascia Mugnozza G, Iori V, Pietrosanti L, Massacci A (2009)
Metal tolerance, accumulation and translocation in poplar and willow clones treated with cadmium in hydroponics. Water Air Soil Pollution 197: 23-34.
CrossRef | Gscholar
Zhou QX (2003)
Interaction between heavy metals and nitrogen fertilizers applied in soil-vegetable systems. Bulletin of Environmental Contamination and Toxicology 71: 388-344.
CrossRef | Gscholar

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