iForest - Biogeosciences and Forestry


Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.

GA Betz (1-2), E Gerstner (1), M Olbrich (1), JB Winkler (3), C Langebartels (1), W Heller (1), H Sandermann (1-4), D Ernst (1)   

iForest - Biogeosciences and Forestry, Volume 2, Issue 3, Pages 114-118 (2009)
doi: https://doi.org/10.3832/ifor0495-002
Published: Jun 10, 2009 - Copyright © 2009 SISEF

Research Articles

Collection/Special Issue: COST Action E52 Meeting 2008 - Florence (Italy)
Evaluation of beech genetic resources for sustainable forestry
Guest Editors: Georg von Wühlisch, Raffaello Giannini

The influence of ozone (150-190 nl L-1; 8h/d) on transcription levels of genes involved in the biosynthesis of the stress hormone ethylene, and its precursor 1-aminocyclopropane-1-carboxylate (ACC), was analysed in leaves of European beech saplings. Ozone-induced leaf lesions appeared 7 weeks after onset of ozone exposure. Cell lesion formation was preceded by persistent increases in ethylene emission, in the level of its malonylated precursor ACC, and in the transcript levels of specific ACC synthase 1 (ACS1), ACS2, ACC oxidase 1 (ACO1), and ACO2. Our results demonstrate that mechanisms similar to those operating in herbaceous plants may determine beech saplings responses to ozone exposure.


Abiotic stress, Ethylene biosynthesis, Fagus sylvatica, Gene expression, Ozone

Authors’ address

GA Betz
E Gerstner
M Olbrich
C Langebartels
W Heller
H Sandermann
D Ernst
Institute of Biochemical Plant Pathology “Helmholtz Zentrum München, German Research Center for Environmental Health, D-85764 Neuherberg (Germany)
GA Betz
Olympus Life Science Research Europa GmbH, Sauerbruchstraße 50, D-81377 München (Germany)
JB Winkler
Department of Environmental Engineering, Helmholtz Zentrum München - German Research Center for Environmental Health, D-85764 Neuherberg (Germany)
H Sandermann
Ecotox Freiburg, Schubertstr. 1, D-79104 Freiburg (Germany)

Corresponding author


Betz GA, Gerstner E, Olbrich M, Winkler JB, Langebartels C, Heller W, Sandermann H, Ernst D (2009). Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.. iForest 2: 114-118. - doi: 10.3832/ifor0495-002

Academic Editor

Marco Borghetti

Paper history

Received: Feb 04, 2009
Accepted: Mar 12, 2009

First online: Jun 10, 2009
Publication Date: Jun 10, 2009
Publication Time: 3.00 months

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List of the papers citing this article based on CrossRef Cited-by.

Betz G, Knappe C, Lapierre C, Olbrich M, Welzl G, Langebartels C, Heller W, Sandermann H, Ernst D (2009a)
Ozone affects shikimate pathway transcripts and monomeric lignin composition in European beech (Fagus sylvatica L.). European Journal of Forest Research 128: 109-116.
CrossRef | Gscholar
Betz AG, Gerstner E, Stich S, Winkler B, Welzl G, Kremmer E, Langebartels C, Heller W, Sandermann H, Ernst D (2009b)
Ozone affects shikimate pathway genes and secondary metabolites of European beech (Fagus sylvatica L.) grown under greenhouse conditions. Trees online first.
CrossRef | Gscholar
Calvo AP, Nicolás C, Lorenzo O, Nicolás G, Rodríguez D (2004)
Evidence for positive regulation by gibberellins and ethylene of ACC oxidase expression and activity during transition from dormancy to germination in Fagus sylvatica L. seeds. Journal of Plant Growth Regulation 23: 44-53.
CrossRef | Gscholar
Corpet F (1988)
Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16: 10881-10890.
CrossRef | Gscholar
Diara C, Castagna A, Baldan B, Sodi AM, Sahr T, Langebartels C, Sebastiani L, Ranieri A (2005)
Differences in the kinetics and scale of signalling molecule production modulate the ozone sensitivity of hybrid poplar clones: the roles of H2O2, ethylene and salicylic acid. New Phytologist 168: 351-364.
CrossRef | Gscholar
Grams TEE, Anegg S, Häberle K-H, Langebartels C, Matyssek R (1999)
Interactions of chronic exposure to elevated CO2 and O3 levels in the photosynthetic light and dark reactions of European beech (Fagus sylvatica). New Phytologist 144: 95-107.
CrossRef | Gscholar
Gupta P, Duplessis S, White H, Karnosky DF, Martin F, Podila GK (2005)
Gene expression patterns of trembling aspen trees following long-term exposure to interacting elevated CO2 and tropospheric O3. New Phytologist 167: 129-142.
CrossRef | Gscholar
Jehnes S, Betz G, Bahnweg G, Haberer K, Sandermann H, Rennenberg H (2007)
Tree internal signalling and defence reactions under ozone exposure in sun and shade leaves of European beech (Fagus sylvatica L.) trees. Plant Biology 9: 253-264.
CrossRef | Gscholar
Kiefer E, Heller W, Ernst D (2000)
A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites. Plant Molecular Biology Reporter 18: 33-39.
CrossRef | Gscholar
Langebartels C, Kangasjärvi J (2004)
Ethylene and jasmonate as regulators of cell death in disease resistance. In: “Molecular Ecotoxicology of Plants” (Sandermann H ed). Springer, Berlin, Germany, pp. 75-109.
Langebartels C, Ernst D, Heller W, Lütz C, Payer H-D, Sandermann H (1997)
Ozone responses of trees: results from controlled chamber exposures at the GSF phytotron. In: “Forest decline and ozone: a comparison of controlled chamber and field experiments” (Sandermann H, Wellburn AR, Heath RL eds). Springer, Berlin, Germany, pp. 163-200.
Langebartels C, Schraudner M, Heller W, Ernst D, Sandermann H (2002)
Oxidative stress and defense reactions in plants exposed to air pollutants and UV-B radiation In: “Oxidative stress in plants” (Inzé D & Van Montagu M eds). Taylor & Francis, London, Great Britain, pp. 105-135.
Matyssek R, Sandermann H (2003)
Impact of ozone on trees: an ecophysiological perspective. Progress in Botany 64: 349-404.
Matyssek R, Bytnerowicz A, Karlsson PE, Paoletti E, Sanz M, Schaub M, Wieser G (2007)
Promoting the O3 flux concept for European forest trees. Environmental Pollution 146: 587-607.
CrossRef | Gscholar
Mehlhorn H, Wellburn AR (1987)
Stress ethylene formation determines plant sensitivity to ozone. Nature 327: 417-418.
CrossRef | Gscholar
Moeder W, Barry CS, Tauriainen AA, Betz C, Tuomainen J, Utriainen M, Grierson D, Sandermann H, Langebartels C, Kangasjärvi J (2002)
Ethylene synthesis regulated by biphasic induction of 1-aminocyclopropane-1-carboxylic acid synthase and 1-aminocyclopropane-1-carboxylic acid oxidase genes is required for hydrogen peroxide accumulation and cell death in ozone-exposed tomato. Plant Physiology 130: 1918-1926.
CrossRef | Gscholar
Nakajima N, Matsuyama T, Tamaoki M, Saji H, Aono M, Kubo A, Kondo N (2001)
Effects of ozone exposure on the gene expression of ethylene biosynthetic enzymes in tomato leaves. Plant Physiology and Biochemistry 39: 993-998.
CrossRef | Gscholar
Nunn AJ, Anegg S, Betz G, Simons S, Kalisch G, Seidlitz HK, Grams TEE, Häberle K-H, Matyssek R, Bahnweg G, Sandermann H, Langebartels C (2005a)
Role of ethylene in the regulation of cell death and leaf loss in ozone-exposed European beech. Plant Cell and Environment 28: 886-897.
CrossRef | Gscholar
Nunn AJ, Kozovits AR, Reiter IM, Heerdt C, Leuchner M, Lütz C, Liu X, Löw M, Winkler JB, Grams TEE, Häberle K-H, Werner H, Fabian P, Rennenberg H, Matyssek R (2005b)
Comparison of ozone uptake and sensitivity between a phytotron study with young beech and a field experiment with adult beech (Fagus sylvatica). Environmental Pollution 137: 494-506.
CrossRef | Gscholar
Olbrich M, Betz G, Gerstner E, Langebartels C, Sandermann H, Ernst D (2005)
Transcriptome analysis of ozone-responsive genes in leaves of European beech (Fagus sylvatica L.). Plant Biology 7: 670-676.
CrossRef | Gscholar
Paoletti E, Ranieri A, Lauteri M (2008)
Moving toward effective ozone flux assessment. Environmental Pollution 156: 16-19.
CrossRef | Gscholar
Rao MV, Lee H, Davis KR (2002)
Ozone-induced ethylene production is dependent on salicylic acid, and both salicylic acid and ethylene act in concert to regulate ozone-induced cell death. The Plant Journal 32: 447-456.
CrossRef | Gscholar
Sandermann H, Ernst D, Heller W, Langebartels C (1998)
Ozone: an abiotic elicitor of plant defence reactions. Trends in Plant Science 3: 47-50.
CrossRef | Gscholar
Schlagnhaufer CD, Arteca RN, Pell EJ (1997)
Sequential expression of two 1-aminocyclopropane-1-carboxylate synthase genes in response to biotic and abiotic stresses in potato (Solanum tuberosum L.) leaves. Plant Molecular Biology 35: 683-688.
CrossRef | Gscholar
Schütt P, Schuck J, Stimm B (1992)
Lexikon der Forstbotanik. Ecomed, Landsberg, Germany.
Tamaoki M, Matsuyama T, Kanna M, Nakajima N, Kubo A, Aono M, Saji H (2003)
Differential ozone sensitivity among Arabidopsis accessions and its relevance to ethylene synthesis. Planta 216: 552-560.
Online | Gscholar
Tuomainen J, Betz C, Kangasjärvi J, Ernst D, Yin Z-H, Langebartels C, Sandermann H (1997)
Ozone induction of ethylene emission in tomato plants: regulation by differential accumulation of transcripts for the biosynthetic enzymes. The Plant Journal 12: 1151-1162.
CrossRef | Gscholar
Vahala J, Ruonala R, Keinänen M, Tuominen H, Kangasjärvi J (2003)
Ethylene insensitivity modulates ozone-induced cell death in birch. Plant Physiology 132: 185-195.
CrossRef | Gscholar

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